A COMBINATION OF LONGSAGE WITH SOLEXA SEQUENCING IS WELL SUITED TO EXPLORE THE DEPTH AND THE COMPLEXITY OF TRANSCRIPTOME

A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome

Abstract Background "Open" transcriptome analysis methods allow to study gene expression without a priori knowledge of the transcript sequences.As of now, SAGE (Serial Analysis of Gene Expression), LongSAGE and MPSS (Massively Parallel Signature Sequencing) are the mostly used methods for "open" transcriptome analysis.Both LongSAGE and MPSS rely on

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Protein and lipid metabolism adjustments in silver catfish (Rhamdia quelen) during different periods of fasting and refeeding

Abstract The fish may experience periods of food deprivation or starvation which produce metabolic changes.In this study, adult Rhamdia quelen males were subjected to fasting periods of 1, 7, 14, and 21 days and of refeeding 2, 4, 6, and 12 c6 corvette door handle days.The results demonstrated that liver protein was depleted after 1 day of fasting,

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